3P Imaging
Three-photon (3P) microscopy enables high-resolution structural and functional imaging deep within tissue, offering improved contrast and axial resolution while supporting calcium imaging of neural activity.
About 3P Imaging
Three-photon microscopy allows noninvasive structural and functional imaging by making cells visible in deep tissues with high spatial resolution and better contrast compared to two-photon excitation. The optical design required for 3P excitation establishes higher axial resolution than that of two-photon microscopy. The spectral window enables three-photon excitation of a variety of fluorophores, such as the current generations of protein-based genetically encoded calcium indicators (e.g. GCaMP6) and the repetition rate of the laser source is adequate for imaging Ca2+ transients produced from neural activity.
The video shows an approximately 1000 μm thick 3P z-stack from a mouse cortex. Purple: blood vessels, green: GFP labelled pericytes. Footage courtesy of Dr. Severin Filser.
Key features of 3P Imaging
2D Multiple-Line Scanning by FEMTOSmart GALVO
Third Harmonic Generation
Advance your research with 3P Imaging
Talk to an application specialist about your research, or book a demo at one of our facilities.