Deep Functional Imaging
Deep functional imaging uses optimized two-photon microscopy to visualize neural activity at depths beyond 1 mm, with performance depending on factors such as laser wavelength, labeling quality, and tissue preparation.
About Deep Functional Imaging
One of the most significant advantages of two-photon microscopy is the ability to examine not only the tissue’s surface but also its deeper layers. While confocal microscopy can penetrate the tissue, it is limited to just a few dozen micrometers.
Thus, a fundamental question arises: How deep can we effectively visualize within the brain?
The answer depends on a multitude of external parameters, including the laser’s wavelength and type, quality of labeling, tissue type, and preparation quality, among others. Furthermore, the imaging solutions themselves play a pivotal role in achieving depths surpassing even 1mm from the surface. In this context, we present our approach to Deep Functional Imaging (DFI).
Key features of Deep Functional Imaging
Optimized Deep-Tissue Excitation
Stable & Automated Beam Conditioning
Optical Efficiency & Sensitive Detection
Extended 3D Focusing Range
Performance at Depth

