FLIM
Two-photon Fluorescence-Lifetime Imaging Microscopy (FLIM) combines deep-tissue imaging with quantitative fluorescence-lifetime measurements to study molecular environments, interactions, and cellular metabolism with reduced photobleaching and minimal sensitivity to signal intensity.
About FLIM — Fluorescence-Lifetime Imaging Microscopy
Fluorescence lifetime is sensitive to the local molecular environment of fluorophores, including polarity, viscosity, and pH, while being generally independent of fluorophore concentration and excitation intensity. Fluorescence Lifetime Imaging (FLIM) reveals these environmental properties, providing insights into molecular interactions and cellular processes or structures. The FLIM Objective Unit enables fluorescence lifetime measurements to explore these parameters in biological samples.
Key features of FLIM
FEMTOSmart Galvo FLIM
Tornado Imaging
FLIM samples
Lifetime trace, tilted: X: -5.9° Y: 4.1°
Advance your research with FLIM
Talk to an application specialist about your research, or book a demo at one of our facilities.