Network Imaging

Network Imaging usecroscopy to studys two-photon mi dynamic neural activity across large 3D networks and multiple cortical layers at high spatiotemporal resolution.

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About Network Imaging

Network Imaging uses two-photon microscopy to study dynamic neural activity across large 3D networks and multiple cortical layers at high spatiotemporal resolution. In other words, complex structures of many individual neurons responding to environmental features, visual or auditory stimuli, reward or punishment, etc. These neural systems extend over 3D space and, in most cases, cross many cortical layers of the brain. With two-photon microscopy enabling neuroscientists to reach these cortical layers (down to 850 µm), it is now possible to study neural networks at a high spatiotemporal resolution.

3D Network Imaging - FEMTO3D Atlas

The FEMTO3D Atlas microscope enables the precise spatial and real-time complexity of neuronal coding to be resolved by scanning large number of cells distributed in a 3D volume.

Imaging of thousands of cells simultaneously
Maximum scanning speed: 100 kHz
High signal-to-noise ratio
Online motion correction even in the axial dimension in behaving animal models
Quasi-simultaneous 3D photostimulation with 3D imaging
All available in the FEMTO3D Atlas two-photon imaging system

2D Network Imaging - FEMTOSmart

FEMTOSmart offers numerous imaging opportunities, and with its vast modularity, researchers can study neuronal networks from varied perspectives and save time in the experimental work.

Scanning Methods

Key features of Network Imaging

3D Random-Access Point

3D Multi-Line

3D Chessboard

3D Multi-Cube scanning method

High-Speed Arbitrary Frame

High-Speed Arbitrary Volume

High-Speed Arbitrary Multi-Plane

Neuronal Network

2D Multiple-Line scanning with FEMTOSmart Galvo

2D FOV & Volume scanning with FEMTOSmart Resonant

Samples

Network Imaging samples

3D Random-Access Point scanning method: Ultra-fast functional sampling of selected points anywhere within a 3D volume.

3D Multi-Line scanning method: High-speed functional imaging along multiple selected lines distributed throughout 3D space.

3D Chessboard scanning method: Parallel functional imaging of hundreds of spatially distributed cells using multiple small frame-based ROIs.

3D Chessboard Scanning in software

3D Multi-Cube scanning method: Motion-tolerant functional imaging using multiple small 3D volumes positioned around selected cells.

High-Speed Arbitrary Frame scanning method: High-speed full-frame raster imaging in freely positioned and tilted planes anywhere in 3D.

High-Speed Arbitrary Volume scanning method: Simultaneous high-speed imaging across multiple independently positioned and non-parallel planes.

High-Speed Arbitrary Multi-Plane scanning method: Fast functional imaging of complete three-dimensional tissue volumes using AO-based multilayer acquisition.

Related publications

Research powered by Femtonics systems

In utero exposure to NMDA receptor autoantibodies disrupts hippocampal circuit maturation

Majoros M, Zhang C, Rahmati V, Graf J, Heckmann M, Geis C, Kreye J, Kaindl AM, Prüss H, Holthoff K, Kirmse K.

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