
Acousto-Optic scanning
No moving parts. Ultrafast scanning. Acousto-optic deflectors steer the laser beam in 3D at up to 100 kHz, letting the Femto3D Atlas record signal from thousands of neurons simultaneously.
About Acousto-Optic (AO) technology
The AO technology uses acousto-optic deflectors to control the position of a laser beam with exceptional speed and precision, without relying on mechanically moving scanning components. This allows the focal point to be repositioned on a microsecond timescale and freely controlled in three dimensions, making the system suitable not only for conventional imaging but also for high-speed 3D measurements and targeted photostimulation. AO-based scanning is particularly advantageous for studying living tissue and fast biological processes, as it provides high spatial and temporal resolution while illuminating only the regions of interest. This helps reduce unnecessary light exposure, photobleaching, and photodamage. As a result, AO technology offers an efficient solution for the real-time, three-dimensional investigation of complex cellular networks and dynamic physiological processes.
Advantages of the Acousto-Optic technology
Single point multiphoton (2P and 3P) excitation
High resolution (preserves maximal 2P and 3P resolution
High volume (~3 cubic mm)
Increased measurement speed
Other advantages
AO technology measurement samples
Latest publications
The methods, explained by the people who use them
webinar
Tune in to the brain - 3D acousto-optic technology in multiphoton microscopy




