Acousto-Optic scanning

Acousto-Optic scanning

No moving parts. Ultrafast scanning. Acousto-optic deflectors steer the laser beam in 3D at up to 100 kHz, letting the Femto3D Atlas record signal from thousands of neurons simultaneously.

About Acousto-Optic (AO) technology

The AO technology uses acousto-optic deflectors to control the position of a laser beam with exceptional speed and precision, without relying on mechanically moving scanning components. This allows the focal point to be repositioned on a microsecond timescale and freely controlled in three dimensions, making the system suitable not only for conventional imaging but also for high-speed 3D measurements and targeted photostimulation. AO-based scanning is particularly advantageous for studying living tissue and fast biological processes, as it provides high spatial and temporal resolution while illuminating only the regions of interest. This helps reduce unnecessary light exposure, photobleaching, and photodamage. As a result, AO technology offers an efficient solution for the real-time, three-dimensional investigation of complex cellular networks and dynamic physiological processes.

Key advantages

Advantages of the Acousto-Optic technology

Single point multiphoton (2P and 3P) excitation

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High resolution (preserves maximal 2P and 3P resolution

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High volume (~3 cubic mm)

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Increased measurement speed

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Other advantages

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Samples

AO technology measurement samples

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Right
Voltage imaging in vitro JEDI-2P measurement combined with uncaging

Voltage imaging in vitro JEDI-2P measurement combined with uncaging

FLIM lifetime trace, tilted: X: -5.9° Y: 4.1°

FLIM lifetime trace, tilted: X: -5.9° Y: 4.1°

Skin of the mouse sole, SHG detected with the upper PMT, merged

Skin of the mouse sole, SHG detected with the upper PMT, merged

Interviews & webinars

The methods, explained by the people who use them

webinar

Tune in to the brain - 3D acousto-optic technology in multiphoton microscopy